The effect of polyphenol to visceral fat profile protein at obesity model rat

Sri Rahayu Lestari, Nuning Wulandari, Siti Imroatul Maslikah


Objective: To asses visceral fat profile protein between normal and obesity model rat. 

Material and Methods: Normal and obesity rat model have been treated with polyphenol from rambutan peel extract for 12 weeks. Rat were divided into 2 major group  based on their weight which normal rat and obesity model rat respectively have average weight 180-200 gram and 360-380 gram. This treatment were divided into minor group which are placebo (without treatment),  treatment with ellagic acid and treatment with polyphenol (dosage 15 mg/kg body weight; 30 mg/kg body weight and 60 mg/kg body weight. Rat were sacrificed after 12 weeks treatment to asses visceral fat profile protein and continue with running SDS PAGE12,5 %. Protein molecular weight sample were calculated with regression analysis between marker protein mobility and logarithm from marker. Band protein analysis were analyze qualitative with SDS PAGE meanwhile quantitative analysis with Gel Doc (Bio Rad). Density band protein were analyze were using Quantity One software and confirmed with Western Blotting.

Result: Protein profile characteristic normal rat and obesity-model rat were in range between117-20 k Da. The amount of band protein were found in normal rat were less than the amount of protein at obesity-model rat. Conclusion: There were difference molecular weight at protein density 57 k Da for obesity model rat which has been treated with rambutan peel extract.


Polyphenol; Obesity model rat; Profil protein

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Albert, B. et al., 2007. Molecular Biology of The Cell 5th ed., Garland Science.

Atkinson, C. et al., 2006. Effects of Cultivar , Fruit Number and Reflected Photosynthetically Active Radiation on Fragaria • ananassa Productivity and Fruit Ellagic Acid and Ascorbic Acid Concentrations. Annals of Botany, 97, pp.429–441.

Ausubel, F.M. et al., 2002. Short Protocol in Molecular Biology, Canada: John Weley and Sons, Ltd.

Kato, H., 2008. Nutrigenomics: the cutting edge and Asian perspectives. Asia Pacific journal of clinical nutrition, 2007;12–5.

Lau, F.C. et al., 2008. Nutrigenomic analysis of diet-gene interactions on functional supplements for weight management. Current genomics. 9:(4):239–51.

Lin, J., Della-Fera, M.A. & Baile, C.A., 2005. Green tea polyphenol epigallocatechin gallate inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Obesity research. 13:(6):982–90.

Ling, L.T. et al., 2010. Assessment of antioxidant capacity and cytotoxicity of selected Malaysian plants. Molecules.15: (4):.2139–51.


Marques, B.G. et al., 2012. Insulin-like growth factor I mediates high-fat diet-induced adipogenesis in Osborne-Mendel rats metabolism regulation Insulin-like growth factor I mediates high-fat diet-induced adipogenesis in Osborne-Mendel rats. Am J Physiol Regul Integr Comp Physiol 278:R254–R662.

Rosen, E.D. et al., 2000. Transcriptional regulation of adipogenesis Transcriptional regulation of adipogenesis. Genes & Development.14: 1293–1307.


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