Antioxidant and hypoglycemic activities of extract and fractions of Rambutan seeds (Nephelium lappaceum L.)

Sylvia Soeng, Endang Evacuasiany, Wahyu Widowati, Nurul Fauziah


Objective : This research was done  to measure the antioxidants and hypoglycemic activities of Nephelium lappaceum L. seed (NLS) extract and fractions.

Methods: The ethanol extract of rambutan seeds were prepared by maseration method and the fractions (n-hexane, ethyl acetate, buthanol and water) by separation of extract based on the polarity. The antioxidant activity was determined by using  superoxide dismutase value (SOD), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. The hypoglycemic activity was determined by using inhibition of α-glucosidase test. The DPPH
scavenging and hypoglycemic activities were analized by median of Inhibitory Concentration (IC-50).
Results: The highest SOD activity showed that ethyl acetate and aqueous fraction of NLS were 3.3771 µg/ml and 3.0374 µg/ml. Meanwhile DPPH assay showed that both NLS extract and fractions had low DPPH scavenging activity. Hypoglycemic activity showed that extract of NLS had highest activity as α-glucosidase inhibitor.

Conclusion: Nephelium lappaceum L. seed extract and fractions have high SOD antioxidant
value but low DPPH scavenging activity and can be used as potential hypoglycemic agent.

Key Words: antidiabetic; antioxidants; α-glucosidase; hypoglycemic; scavenging

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