Protein extract of Detam 1 soybean contains β–conglycinin stimulating cholecystokinin signal transduction through activities of PKC, c-RAF and ERK 1/2 in Wistar rats

Melinah Hidayat

Abstract


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Protein extract of Detam 1 soybean contains β–conglycinin stimulating
cholecystokinin signal transduction through activities of PKC, c-RAF and ERK
1/2 in Wistar rats
Meilinah Hidayat1†, Ahmad Faried2
1Nutrition Department Faculty of Medicine, Maranatha Christian University Bandung
2Research and Development Unit Faculty of Medicine, Padjadjaran University, Bandung
A R T I C L E I N F O
Article history:
Received: February 2015
Accepted: June 2015
Available online:
August 2015
Keywords:
β conglycinin
Cholecystokinin
Protein Extract of Detam 1
soybean
Signal transduction
†Corresponding author:
Nutrition Department
Faculty of Medicine,
Maranatha Christian
University Bandung
E-mail:
mellahidayat@yahoo.com
A B S T R A C T
1. Introduction
The prevalence of obesity is definitely
increasing and is widely spread in all over the
world (1). This condition should be overcome
because obesity can cause many side effects. One
of options solution is to discover good nutrition
sources that can reduce body weight. Soybean
(Glycine Max L. Merr) is a good source of
protein. It has two major protein fractions, β-
conglycinin (7S) and glycinin (11S) (2). β -
conglycinin’s function was known to suppress
food intake, decreasing appetite (3) reducing body
weight and this effect may be due to stimulating
gastrointestinal hormones, cholecystokinin (CCK)
release (4). CCK is a peptide hormone of the
gastrointestinal system that can act as a short
term food intake inhibitor, has been
demonstrated to reduce food intake in a doserelated
manner in different species and
experimental designs (5, 6, 7, 8).
Protein can stimulate CCK secretion in STC-1
cells duodenum through signal transduction of G
Protein Coupling Receptor (GPCR) mechanisms
(9). There are many possibilities in the pathway,
such as Protein Kinase C (PKC), DiAcyl Glycerol
(DAG), Protein Kinase (PKA), c-Raf and
Extracellular Signal-Related Kinase (ERK) or
Mitogen Activated Protein Kinase (MAPK) (9).
However, the cellular mechanisms by which the
β-conglycinin induces CCK secretion remain to be
clarified.
There hasn’t been many studies about
Indonesian soybean variety in the relation to CCK
Objective: to determine which kind of soybean extract contains the highest β-conglycinin level, their effects to
cholecystokinin (CCK) plasma level, and to signal transduction pathway in duodenum of Wistar rats.
Methods: β-conglycinin were analysed in vitro using sodium dodecyl sulphate polyacrylamide gel
electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The CCK plasma level were measured
in vivo in 24 male Wistar rats by ELISA method, using t-test paired and the activity of PKCμ, PKA, c-Raf and ERK
in CCK signal transduction pathway in vivo by Western blot method and Scion image densitometry (DU)
analysed using ANOVA.
Result: Protein extract of Detam 1 soybean (PEDS) contains the highest amount of β-conglycinin level. There
was no significant diffrerence of CCK plasma level between pre and post treatment (p> 0.05) although there were
increasing level in all treatment. The duodenum of Wistar rats which were given PEDS showed significant
difference with negative control in signal transduction activities of PKCμ, c-Raf & ERK 1/2 pathway (p<0.001).
Conclusion: PEDS contains the highest β-conglycinin level, effects to CCK plasma level, and to CCK signal
transduction pathway through activities of PKCμ, c-Raf and ERK 1/2 in enteroendocrine cells in duodenum
Wistar rats

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