Effect of Folic Acid and Vitamin B12 Supplementation on Homocysteine Level in association with MTHFR C677T Polymorphisms in Overweight Female Adults

Fidelia Fiddelia

Abstract


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Effect of Folic Acid and Vitamin B12 Supplementation on Homocysteine Level
in association with MTHFR C677T Polymorphisms in Overweight Female
Adults
Fidelia Fidelia1, Antonius Suwanto1, Felicia Kartawidjajaputra2†, Susana Susana2
1 Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta
2Bioanalytical Laboratory Nutrifood Research Center, PT Nutrifood Indonesia, Jakarta
A R T I C L E I N F O
Article history:
Received: February 2015
Accepted: June 2015
Available online:
August 2015
Keywords:
Cardiovascular disease
Folate
Homocysteine
Methylenetetrahydrofolate
reductase C677T
Vitamin B12
†Corresponding author:
Bioanalytical Laboratory
Nutrifood Research Center,
PT Nutrifood Indonesia,
Jakarta
E-mail:
felicia@nutrifood.co.id
A B S T R A C T
1. Introduction
Cardiovascular disease (CVD) is known as the
leading cause of death and disability worldwide,
including Indonesia. In 2008, an estimated of 17.3
million people died from CVDs and by 2030, it is
predicted to be risen over 23 million people.
Atherosclerotic heart disease, or widely known as
coronary heart disease (CHD) is one of CVD
group occupied the first place of cause of death in
Indonesia and the number keeps rising over the
years [1-3].
There are many factors associated with
increased risk of CVD. Some of risk factors are
classified as non-modifiable, such as age, sex and
genetics; whereas behavioral risk factors, such as
physical inactivity, tobacco use, unhealthy diet,
and harmful use of alcohol, which are responsible
for about 80% of atherosclerotic heart disease, are
able to be modified by lifestyle or pharmaceutical
intervention. Both of modifiable and nonmodifiable
factors can lead to intermediate risk
factors, such as hypertension, hyperlipidemia,
glucose intolerance and overweight or obesity,
which can lead to chronic disease development
[4].
80-90% of people dying from CHD have one or
more major risk factors that are influenced by
lifestyle. Among the numerous factors above,
overweight is one to be highlighted especially
because the incidence is increasing each year.
World Health Organization stated that the
prevalence of overweight in Indonesian women is
Objective: The aim of this study was to evaluate the effect of folic acid and vitamin B12 supplementation on Hcy
level based on the MTHFR C677T polymorphisms in Indonesian overweight females.
Methods: The study involved 110 female adults (20±1.3 years) with a body mass index (BMI) above 22.9 (mean:
25.3±2.3). DNA was extracted from saliva in order to identify the SNP of MTHFRC677T using PCR-RFLP. 15 of 110
subjects were chosen to represent all MTHFR genotypes (5 CC, 6 CT and 4 TT) and assigned two different
treatments: control (placebo), and supplement (500μg folic acid and 1 mg vitamin B12) for 4 weeks. Hcy levels
were measured using direct immunochemical assay.
Result: This study population (n=110) showed that 677CC allele had the highest frequency (67%), followed by
677CT (29%) and 677TT (4%). The results demonstrated no significant differences of Hcy level among all
subjects before treatment, but Hcy level was slightly higher in T allele subjects. The folate and vitamin B12
treatment significantly reduced the level of Hcy in supplement group (p = 0.038), but not in placebo group. The
difference between placebo and treatment was observed to be the highest in TT, followed by CT and CC subjects.
Conclusion: This study showed that subjects who had T allele, tended to have a higher level of Hcy.
Supplementation of folate and vitamin B12 was proven to lower the level of Hcy, and TT subjects were proven to
be the most responsive.

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